{"id":8988,"date":"2025-12-30T15:42:22","date_gmt":"2025-12-30T07:42:22","guid":{"rendered":"https:\/\/www.akademisains.gov.my\/asmsj\/?post_type=article&#038;p=8988"},"modified":"2025-12-30T15:42:22","modified_gmt":"2025-12-30T07:42:22","slug":"sea-urchin-diadema-setosum-chocolate-snack-on-haemoglobin-body-weight-and-brain-characteristics-in-mice-mus-musculus","status":"publish","type":"article","link":"https:\/\/www.akademisains.gov.my\/asmsj\/article\/sea-urchin-diadema-setosum-chocolate-snack-on-haemoglobin-body-weight-and-brain-characteristics-in-mice-mus-musculus\/","title":{"rendered":"Sea Urchin (Diadema setosum) Chocolate Snack on Haemoglobin, Body Weight, and Brain Characteristics in Mice (Mus musculus)"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row][vc_column width=&#8221;4\/5&#8243; css=&#8221;.asmsj-main-column{padding-bottom:10px !important;}&#8221;][vc_row_inner equal_height=&#8221;yes&#8221; css=&#8221;.asmsj-inner-row{margin-bottom:10px !important;}&#8221;][vc_column_inner][vc_column_text css=&#8221;&#8221;]\t\t\r\n\t\t<div class=\"mdocs\">\r\n\t\t\t<div class=\"mdocs-wrap\"><\/div>\r\n\t\t\t<div class=\"mdocs-container\">\r\n\t\t\t\t<table class=\"table table-hover table-condensed\" id=\"mdocs-list-table\">\r\n\t\t\t\t\t<thead>\r\n\t\t\t\t\t\t<tr>\r\n\t\t\t\t\t\t\t<th class=\"mdocs-name\">Name<\/th>\r\n\t\t\t\t\t\t\t<th class=\"mdocs-downloads\">Downloads<\/th>\r\n\t\t\t\t\t\t\t<th class=\"mdocs-version\">Version<\/th>\r\n\t\t\t\t\t\t\t<th class=\"mdocs-author\">Owner<\/th>\r\n\t\t\t\t\t\t\t<th class=\"mdocs-modified\">Last Updated<\/th>\r\n\t\t\t\t\t\t\t<th class=\"mdocs-file-size\">File Size<\/th>\r\n\t\t\t\t\t\t<\/tr>\r\n\t\t\t\t\t<\/thead>\r\n\t\t\t\t\t<tbody>\r\n\t\t\t\t\t\t<tr class=\"mdocs-normal\">\r\n\t\t\t\t\t\t\t<td class=\"mdocs-name\">\r\n\t\t\t\t\t\t\t\t<div class=\"mdocs-btn-group btn-group\">\r\n\t\t\t\t\t\t\t\t\t<a class=\"mdocs-title-href\" href=\"https:\/\/www.akademisains.gov.my\/asmsj\/?sdm_process_download=1&#038;download_id=8987\" target=\"_blank\">\r\n\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" src=\"https:\/\/www.akademisains.gov.my\/asmsj\/wp-content\/plugins\/simple-download-monitor\/images\/pdf.png\" class=\"mdoc-file-type-icon\" alt=\"PDF\">\r\n\t\t\t\t\t\t\t\t\t\tSea Urchin (Diadema setosum) Chocolate Snack on Haemoglobin, Body Weight, and Brain Characteristics in Mice (Mus musculus)\t\t\t\t\t\t\t\t\t<\/a>\r\n\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t<\/td>\r\n\t\t\t\t\t\t\t<td class=\"mdocs-downloads\">\r\n\t\t\t\t\t\t\t\t<i class=\"fas fa-cloud-download-alt\" aria-hidden=\"true\" title=\"Downloads\"><\/i>\r\n\t\t\t\t\t\t\t\t<em style=\"color: #FF9140;\">150 <small>downloads<\/small><\/em>\r\n\t\t\t\t\t\t\t<\/td>\r\n\t\t\t\t\t\t\t<td class=\"mdocs-version\">\r\n\t\t\t\t\t\t\t\t<i class=\"fa fa-history\" aria-hidden=\"true\" title=\"Version\"><\/i>\r\n\t\t\t\t\t\t\t\t<em style=\"color: #0066FF;\">1.0<\/em>\r\n\t\t\t\t\t\t\t<\/td>\r\n\t\t\t\t\t\t\t<td class=\"mdocs-author\">\r\n\t\t\t\t\t\t\t\t<i class=\"fa fa-user\" aria-hidden=\"true\" title=\"Owner\"><\/i>\r\n\t\t\t\t\t\t\t\t<em style=\"color: #666;\">ikram<\/em>\r\n\t\t\t\t\t\t\t<\/td>\r\n\t\t\t\t\t\t\t<td class=\"mdocs-modified\">\r\n\t\t\t\t\t\t\t\t<i class=\"fa fa-calendar\" aria-hidden=\"true\" title=\"Last Modified\"><\/i>\r\n\t\t\t\t\t\t\t\t<em style=\"color: #990000;\">30-12-2025 15:33<\/em>\r\n\t\t\t\t\t\t\t<\/td>\r\n\t\t\t\t\t\t\t<td class=\"mdocs-file-size\">\r\n\t\t\t\t\t\t\t\t<i class=\"fa fa-database\" aria-hidden=\"true\" title=\"File Size\"><\/i>\r\n\t\t\t\t\t\t\t\t<em>407 KB<\/em>\r\n\t\t\t\t\t\t\t<\/td>\r\n\t\t\t\t\t\t<\/tr>\r\n\t\t\t\t\t<\/tbody>\r\n\t\t\t\t<\/table>\r\n\t\t\t<\/div>\r\n\t\t<\/div>\r\n\r\n\t\t<div class=\"vc_btn3-container vc_btn3-center vc_custom_1753429848041 vc_do_btn\">\r\n\t\t\t<a class=\"vc_general vc_btn3 vc_btn3-size-md vc_btn3-shape-square vc_btn3-style-flat vc_btn3-block vc_btn3-icon-left vc_btn3-color-grey\" \r\n\t\t\t   href=\"https:\/\/www.akademisains.gov.my\/asmsj\/?sdm_process_download=1&#038;download_id=8987\" target=\"_blank\">\r\n\t\t\t\t<i class=\"vc_btn3-icon \"><\/i> Download PDF\r\n\t\t\t<\/a>\r\n\t\t<\/div>\r\n\t\t\t\r\n    <div class=\"asmsj-article-header\">\r\n        <p>ASM Sc. J., 20(2), 2025<\/p>\r\n\t\t<p>Published on December 30, 2025<\/p>\r\n                    <p><a href=\"https:\/\/doi.org\/10.32802\/asmscj.2025.1701\" target=\"_blank\" rel=\"noopener\">https:\/\/doi.org\/10.32802\/asmscj.2025.1701<\/a><\/p>\r\n        \r\n                    <p><strong>Author: <\/strong>Azahra Aliyyu Denaldo, Ahmad Syauqy, Delianis Pringgenies<\/p>\r\n        \r\n                    <p><strong>Abstract<\/strong><\/p>\r\n\t\t\t<p style=\"text-align:justify;\">This study investigated the effects of Diadema setosum gonad-based chocolate products on amino acid content, body weight, haemoglobin levels, neuronal cell count, brain weight, and brain volume in Mus musculus. A particular focus was placed on evaluating neurodevelopmental impacts and nutritional benefits.  Amino  acid  composition  of  the  D.  setosum gonad  product  was  analysed  using  high- performance liquid chromatography (HPLC). Male rats were divided into groups receiving 0.5 g, 1.0 g, or 1.5 g\/subject\/day of gonad chocolate, with or without fish oil, for 70 days. Measurements included body weight, haemoglobin level, brain weight and volume, and neuronal cell count via histological analysis. The gonad product contained high levels of alanine, glutamic acid, and glycine. The highest body weight increase occurred in the negative control group (39.4 g). The highest haemoglobin level was recorded in the P2 group (13.15 g\/dL), which received 1.0 g\/day of the gonad product. Brain weight peaked in the negative control group (0.485 g), while the highest brain volume was in the P3 group (3.25 mL). The highest neuronal cell count was also observed in P2 (838 cells). ANOVA results showed no significant differences in body weight, haemoglobin, brain weight, or brain volume. However, a substantial increase was found in neuronal cell count (p &lt; 0.05). Conclusion: Administration of 1.0 g\/day D. setosum gonad chocolate significantly increased haemoglobin levels and neuronal cell count in rats, suggesting potential neuroprotective and haematological benefits. Further research is warranted to explore its applicability in human nutrition and cognitive health.<\/p>\r\n        \r\n                    <p><strong>Keywords:<\/strong>\r\n                amino acid, gonad, HPLC, neuron cell, nutrition            <\/p>\r\n            <\/div>\r\n\t\r\n\t\r\n\t\r\n\t<div class=\"asmsj-citation-box\">\r\n        <h3>How to Cite<\/h3>\r\n        <p id=\"citation-text\">\r\n            2025. Sea Urchin (Diadema setosum) Chocolate Snack on Haemoglobin, Body Weight, and Brain Characteristics in Mice (Mus musculus). <em>ASM Science Journal<\/em>, 20(2), 1-9. <a href=\"https:\/\/doi.org\/10.32802\/asmscj.2025.1701\" target=\"_blank\" rel=\"noopener\">https:\/\/doi.org\/10.32802\/asmscj.2025.1701<\/a>        <\/p>\r\n\r\n        <div class=\"asmsj-actions\">\r\n            <select id=\"citation-format\">\r\n                <option value=\"apa\">APA<\/option>\r\n                <option value=\"mla\">MLA<\/option>\r\n                <option value=\"chicago\">Chicago<\/option>\r\n                <option value=\"harvard\">Harvard<\/option>\r\n                <option value=\"ieee\">IEEE<\/option>\r\n                <option value=\"vancouver\">Vancouver<\/option>\r\n            <\/select>\r\n\r\n            <button id=\"copy-citation\">Copy<\/button>\r\n            <div id=\"loading-spinner\" class=\"hidden\"><\/div>\r\n        <\/div>\r\n    <\/div>\r\n\r\n    <script>\r\n    jQuery(document).ready(function($) {\r\n        $('#citation-format').change(function() {\r\n            var format = $(this).val();\r\n            var postID = 8988;\r\n            \r\n            $('#loading-spinner').show(); \/\/ Show loading animation\r\n\r\n            $.post(asmsj_ajax.ajaxurl, {\r\n                action: 'generate_citation',\r\n                format: format,\r\n                post_id: postID\r\n            }, function(response) {\r\n                $('#citation-text').html(response);\r\n                $('#loading-spinner').hide(); \/\/ Hide loading animation\r\n            });\r\n        });\r\n\r\n        $('#copy-citation').click(function() {\r\n            var citation = $('#citation-text').text();\r\n            navigator.clipboard.writeText(citation).then(function() {\r\n                alert(\"Citation copied to clipboard!\");\r\n            });\r\n        });\r\n    });\r\n    <\/script>\r\n    [\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_column][vc_column width=&#8221;1\/5&#8243;][vc_widget_sidebar sidebar_id=&#8221;sidebar-blog&#8221;][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>Azahra Aliyyu Denaldo, Ahmad Syauqy, Delianis Pringgenies<br \/><a href='https:\/\/doi.org\/10.32802\/asmscj.2025.1701'>https:\/\/doi.org\/10.32802\/asmscj.2025.1701<\/a><\/p>\n","protected":false},"author":827,"featured_media":0,"menu_order":0,"comment_status":"open","ping_status":"open","template":"","format":"standard","meta":{"footnotes":""},"categories":[73],"tags":[3978,3979,811,3980,3981],"issuem_issue":[3806],"class_list":["post-8988","article","type-article","status-publish","format-standard","hentry","category-research-articles","tag-amino-acid","tag-gonad","tag-hplc","tag-neuron-cell","tag-nutrition","issuem_issue-asm-sc-j-vol-20-no-2-2025"],"jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/www.akademisains.gov.my\/asmsj\/wp-json\/wp\/v2\/article\/8988","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.akademisains.gov.my\/asmsj\/wp-json\/wp\/v2\/article"}],"about":[{"href":"https:\/\/www.akademisains.gov.my\/asmsj\/wp-json\/wp\/v2\/types\/article"}],"author":[{"embeddable":true,"href":"https:\/\/www.akademisains.gov.my\/asmsj\/wp-json\/wp\/v2\/users\/827"}],"replies":[{"embeddable":true,"href":"https:\/\/www.akademisains.gov.my\/asmsj\/wp-json\/wp\/v2\/comments?post=8988"}],"version-history":[{"count":2,"href":"https:\/\/www.akademisains.gov.my\/asmsj\/wp-json\/wp\/v2\/article\/8988\/revisions"}],"predecessor-version":[{"id":8991,"href":"https:\/\/www.akademisains.gov.my\/asmsj\/wp-json\/wp\/v2\/article\/8988\/revisions\/8991"}],"wp:attachment":[{"href":"https:\/\/www.akademisains.gov.my\/asmsj\/wp-json\/wp\/v2\/media?parent=8988"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.akademisains.gov.my\/asmsj\/wp-json\/wp\/v2\/categories?post=8988"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.akademisains.gov.my\/asmsj\/wp-json\/wp\/v2\/tags?post=8988"},{"taxonomy":"issuem_issue","embeddable":true,"href":"https:\/\/www.akademisains.gov.my\/asmsj\/wp-json\/wp\/v2\/issuem_issue?post=8988"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}