{"id":9340,"date":"2026-10-02T15:18:43","date_gmt":"2026-10-02T07:18:43","guid":{"rendered":"https:\/\/www.akademisains.gov.my\/asmsj\/?post_type=article&#038;p=9340"},"modified":"2026-10-02T15:18:43","modified_gmt":"2026-10-02T07:18:43","slug":"characterisation-of-amylase-produced-by-local-isolate-aspergillus-flavus-nsh9-undergone-solid-state-fermentation-on-rice-husks","status":"publish","type":"article","link":"https:\/\/www.akademisains.gov.my\/asmsj\/article\/characterisation-of-amylase-produced-by-local-isolate-aspergillus-flavus-nsh9-undergone-solid-state-fermentation-on-rice-husks\/","title":{"rendered":"Characterisation of Amylase Produced by Local Isolate Aspergillus flavus NSH9 Undergone Solid-state Fermentation on Rice Husks"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row][vc_column width=&#8221;4\/5&#8243; css=&#8221;.asmsj-main-column{padding-bottom:10px !important;}&#8221;][vc_row_inner equal_height=&#8221;yes&#8221; css=&#8221;.asmsj-inner-row{margin-bottom:10px !important;}&#8221;][vc_column_inner][vc_column_text css=&#8221;&#8221;]\t\t\r\n\t\t<div class=\"mdocs\">\r\n\t\t\t<div class=\"mdocs-wrap\"><\/div>\r\n\t\t\t<div class=\"mdocs-container\">\r\n\t\t\t\t<table class=\"table table-hover table-condensed\" id=\"mdocs-list-table\">\r\n\t\t\t\t\t<thead>\r\n\t\t\t\t\t\t<tr>\r\n\t\t\t\t\t\t\t<th class=\"mdocs-name\">Name<\/th>\r\n\t\t\t\t\t\t\t<th class=\"mdocs-downloads\">Downloads<\/th>\r\n\t\t\t\t\t\t\t<th class=\"mdocs-version\">Version<\/th>\r\n\t\t\t\t\t\t\t<th class=\"mdocs-author\">Owner<\/th>\r\n\t\t\t\t\t\t\t<th class=\"mdocs-modified\">Last Updated<\/th>\r\n\t\t\t\t\t\t\t<th class=\"mdocs-file-size\">File Size<\/th>\r\n\t\t\t\t\t\t<\/tr>\r\n\t\t\t\t\t<\/thead>\r\n\t\t\t\t\t<tbody>\r\n\t\t\t\t\t\t<tr class=\"mdocs-normal\">\r\n\t\t\t\t\t\t\t<td class=\"mdocs-name\">\r\n\t\t\t\t\t\t\t\t<div class=\"mdocs-btn-group btn-group\">\r\n\t\t\t\t\t\t\t\t\t<a class=\"mdocs-title-href\" href=\"https:\/\/www.akademisains.gov.my\/asmsj\/?sdm_process_download=1&#038;download_id=9338\" target=\"_blank\">\r\n\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" src=\"https:\/\/www.akademisains.gov.my\/asmsj\/wp-content\/plugins\/simple-download-monitor\/images\/pdf.png\" class=\"mdoc-file-type-icon\" alt=\"PDF\">\r\n\t\t\t\t\t\t\t\t\t\tCharacterisation of Amylase Produced by Local Isolate Aspergillus flavus NSH9 Undergone Solid-state Fermentation on Rice Husks\t\t\t\t\t\t\t\t\t<\/a>\r\n\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t<\/td>\r\n\t\t\t\t\t\t\t<td class=\"mdocs-downloads\">\r\n\t\t\t\t\t\t\t\t<i class=\"fas fa-cloud-download-alt\" aria-hidden=\"true\" title=\"Downloads\"><\/i>\r\n\t\t\t\t\t\t\t\t<em style=\"color: #FF9140;\">23 <small>downloads<\/small><\/em>\r\n\t\t\t\t\t\t\t<\/td>\r\n\t\t\t\t\t\t\t<td class=\"mdocs-version\">\r\n\t\t\t\t\t\t\t\t<i class=\"fa fa-history\" aria-hidden=\"true\" title=\"Version\"><\/i>\r\n\t\t\t\t\t\t\t\t<em style=\"color: #0066FF;\">1.0<\/em>\r\n\t\t\t\t\t\t\t<\/td>\r\n\t\t\t\t\t\t\t<td class=\"mdocs-author\">\r\n\t\t\t\t\t\t\t\t<i class=\"fa fa-user\" aria-hidden=\"true\" title=\"Owner\"><\/i>\r\n\t\t\t\t\t\t\t\t<em style=\"color: #666;\">ikram<\/em>\r\n\t\t\t\t\t\t\t<\/td>\r\n\t\t\t\t\t\t\t<td class=\"mdocs-modified\">\r\n\t\t\t\t\t\t\t\t<i class=\"fa fa-calendar\" aria-hidden=\"true\" title=\"Last Modified\"><\/i>\r\n\t\t\t\t\t\t\t\t<em style=\"color: #990000;\">02-10-2026 15:00<\/em>\r\n\t\t\t\t\t\t\t<\/td>\r\n\t\t\t\t\t\t\t<td class=\"mdocs-file-size\">\r\n\t\t\t\t\t\t\t\t<i class=\"fa fa-database\" aria-hidden=\"true\" title=\"File Size\"><\/i>\r\n\t\t\t\t\t\t\t\t<em>612 KB<\/em>\r\n\t\t\t\t\t\t\t<\/td>\r\n\t\t\t\t\t\t<\/tr>\r\n\t\t\t\t\t<\/tbody>\r\n\t\t\t\t<\/table>\r\n\t\t\t<\/div>\r\n\t\t<\/div>\r\n\r\n\t\t<div class=\"vc_btn3-container vc_btn3-center vc_custom_1753429848041 vc_do_btn\">\r\n\t\t\t<a class=\"vc_general vc_btn3 vc_btn3-size-md vc_btn3-shape-square vc_btn3-style-flat vc_btn3-block vc_btn3-icon-left vc_btn3-color-grey\" \r\n\t\t\t   href=\"https:\/\/www.akademisains.gov.my\/asmsj\/?sdm_process_download=1&#038;download_id=9338\" target=\"_blank\">\r\n\t\t\t\t<i class=\"vc_btn3-icon \"><\/i> Download PDF\r\n\t\t\t<\/a>\r\n\t\t<\/div>\r\n\t\t\t\r\n    <div class=\"asmsj-article-header\">\r\n        <p>ASM Sc. J., 21(2), 2026<\/p>\r\n\t\t<p>Published on October 2, 2026<\/p>\r\n                    <p><a href=\"https:\/\/doi.org\/10.32802\/asmscj.2026.209\" target=\"_blank\" rel=\"noopener\">https:\/\/doi.org\/10.32802\/asmscj.2026.209<\/a><\/p>\r\n        \r\n                    <p><strong>Author: <\/strong>Rosmawati Saat, Mohd Hasnain Hussain, Awang Ahmad Sallehin Husaini<\/p>\r\n        \r\n                    <p><strong>Abstract<\/strong><\/p>\r\n\t\t\t<p style=\"text-align:justify;\">Amylases are a group of important hydrolytic enzymes that ha ve been applied in a vast variety of industries and processes around the world.  Amongst the different hydrolases, amylases possess the highest potential for use of biopharmaceutical and medicinal purposes. These starch -degrading enzymes are omnipresent glycoside hydrolases that can be found in a variety of organisms which include animals, plants, a few yeasts, bacteria and fungi. However, fungi have been shown to possess a list of advantages over other organisms in producing amylase. Mesophilic fungi such as Aspergillus spp. are reported to be one of the dominant amylase-producing species. Despite many species of Aspergillus  have  been  identified,  only  two  species  have  been  extensively  studied  and  applied commercially for amylase production. Thus, amylase obtained from a local isolate Aspergillus flavus NSH9 which undergone solid-state fermentation on rice husks without supplement was studied as an attempt to outsource potential local industrial competent amylases. Biochemical analyses such as effects of temperature, pH, incubation period, metal ions, soluble starch concentration and different reagents were performed as to investigate the nature of the enzyme. Also, molecular analyses were conducted which include Native-PAGE, SDS-PAGE and zymography to identify the types of amylases present.<\/p>\r\n        \r\n                    <p><strong>Keywords:<\/strong>\r\n                amylase, Aspergillus flavus, biochemical analyses, molecular analyses, solid-state fermentation            <\/p>\r\n            <\/div>\r\n\t\r\n\t\r\n\t\r\n\t<div class=\"asmsj-citation-box\">\r\n        <h3>How to Cite<\/h3>\r\n        <p id=\"citation-text\">\r\n            2026. Characterisation of Amylase Produced by Local Isolate Aspergillus flavus NSH9 Undergone Solid-state Fermentation on Rice Husks. <em>ASM Science Journal<\/em>, 21(2), 1-15. <a href=\"https:\/\/doi.org\/10.32802\/asmscj.2026.209\" target=\"_blank\" rel=\"noopener\">https:\/\/doi.org\/10.32802\/asmscj.2026.209<\/a>        <\/p>\r\n\r\n        <div class=\"asmsj-actions\">\r\n            <select id=\"citation-format\">\r\n                <option value=\"apa\">APA<\/option>\r\n                <option value=\"mla\">MLA<\/option>\r\n                <option value=\"chicago\">Chicago<\/option>\r\n                <option value=\"harvard\">Harvard<\/option>\r\n                <option value=\"ieee\">IEEE<\/option>\r\n                <option value=\"vancouver\">Vancouver<\/option>\r\n            <\/select>\r\n\r\n            <button id=\"copy-citation\">Copy<\/button>\r\n            <div id=\"loading-spinner\" class=\"hidden\"><\/div>\r\n        <\/div>\r\n    <\/div>\r\n\r\n    <script>\r\n    jQuery(document).ready(function($) {\r\n        $('#citation-format').change(function() {\r\n            var format = $(this).val();\r\n            var postID = 9340;\r\n            \r\n            $('#loading-spinner').show(); \/\/ Show loading animation\r\n\r\n            $.post(asmsj_ajax.ajaxurl, {\r\n                action: 'generate_citation',\r\n                format: format,\r\n                post_id: postID\r\n            }, function(response) {\r\n                $('#citation-text').html(response);\r\n                $('#loading-spinner').hide(); \/\/ Hide loading animation\r\n            });\r\n        });\r\n\r\n        $('#copy-citation').click(function() {\r\n            var citation = $('#citation-text').text();\r\n            navigator.clipboard.writeText(citation).then(function() {\r\n                alert(\"Citation copied to clipboard!\");\r\n            });\r\n        });\r\n    });\r\n    <\/script>\r\n    [\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_column][vc_column width=&#8221;1\/5&#8243;][vc_widget_sidebar sidebar_id=&#8221;sidebar-blog&#8221;][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>Rosmawati Saat, Mohd Hasnain Hussain, Awang Ahmad Sallehin Husaini<br \/>https:\/\/doi.org\/10.32802\/asmscj.2026.209<\/a><\/p>\n","protected":false},"author":827,"featured_media":0,"menu_order":0,"comment_status":"open","ping_status":"open","template":"","format":"standard","meta":{"footnotes":""},"categories":[73],"tags":[3944,3941,4166,4167,3943],"issuem_issue":[4111],"class_list":["post-9340","article","type-article","status-publish","format-standard","hentry","category-research-articles","tag-amylase","tag-aspergillus-flavus","tag-biochemical-analyses","tag-molecular-analyses","tag-solid-state-fermentation","issuem_issue-asm-sc-j-vol-21-no-2-2026"],"jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/www.akademisains.gov.my\/asmsj\/wp-json\/wp\/v2\/article\/9340","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.akademisains.gov.my\/asmsj\/wp-json\/wp\/v2\/article"}],"about":[{"href":"https:\/\/www.akademisains.gov.my\/asmsj\/wp-json\/wp\/v2\/types\/article"}],"author":[{"embeddable":true,"href":"https:\/\/www.akademisains.gov.my\/asmsj\/wp-json\/wp\/v2\/users\/827"}],"replies":[{"embeddable":true,"href":"https:\/\/www.akademisains.gov.my\/asmsj\/wp-json\/wp\/v2\/comments?post=9340"}],"version-history":[{"count":3,"href":"https:\/\/www.akademisains.gov.my\/asmsj\/wp-json\/wp\/v2\/article\/9340\/revisions"}],"predecessor-version":[{"id":9343,"href":"https:\/\/www.akademisains.gov.my\/asmsj\/wp-json\/wp\/v2\/article\/9340\/revisions\/9343"}],"wp:attachment":[{"href":"https:\/\/www.akademisains.gov.my\/asmsj\/wp-json\/wp\/v2\/media?parent=9340"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.akademisains.gov.my\/asmsj\/wp-json\/wp\/v2\/categories?post=9340"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.akademisains.gov.my\/asmsj\/wp-json\/wp\/v2\/tags?post=9340"},{"taxonomy":"issuem_issue","embeddable":true,"href":"https:\/\/www.akademisains.gov.my\/asmsj\/wp-json\/wp\/v2\/issuem_issue?post=9340"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}